England boss Gareth Southgate printed the national anthem and called on his Three Lions stars to “sing with pride”.
Wembley will have 45,000 fans in attendance for the last 16 clashes against Germany on Tuesday night.
Southgate believes that a powerful rendition of the anthem could give them the edge early on.
Ahead of England’s Euro 96 semi-final against Germany, former Wembley players and fans sang ‘God Save the Queen’ in what has become an iconic moment.
Ahead of last week’s 1-0 win over the Czech Republic, TV cameras showed all members of England’s starting XI singing the anthem.
Dominic Calvert-Lewin started off the bench for the Group D victory but revealed he got goosebumps when he sang it with fans in attendance.
He said, “It’s amazing. My first experience playing for England with fans was the friendly against Austria this month.
“It was the first time I heard the national anthem as a player and got to sing it as a player with fans in the stadium – it gave me goosebumps.
“So playing the Euro, with some 40,000 chants in the stadium, it’s going to make a huge difference.
“It gives you an extra boost and it’s just a very proud moment.”
Southgate is certainly a patriotic individual and has sought to unite the team and his fans since taking the post in England.
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The 50-year-old hammered his players that they would be in the spotlight when it came to singing the national anthem and it was up to them to underline their pride in playing for England.
A source told The Sun: “In previous tournaments players have been seen grudgingly mumbling the words – some even left their mouths speechless.
“Gareth is extremely patriotic and believes it is essential for players to develop a sense of national pride by learning and singing the words.
“He had the players sit down before the tournament and explained to them that the cameras would be on them all the time and that it was essential to show the fans what it means to wear an English shirt.
“If the players sing, it inspires the crowd and those who watch at home.”
The source added: “There have been a few ribs for the more deaf players, but it’s great for team spirit.”
England were eliminated in majors by Germany in 2010 and 1996.
They enter the game as close favorites, however, and would face either Ukraine or Sweden if they reach the bottom eight.